摘要
目的 探讨急性髓系白血病 (AML)中Evi1基因的表达及意义。方法 应用半定量RT -PCR方法检测了HEL白血病细胞系 (Evi1阳性细胞株 )、94例AML(初治 49例 ,复发 2 3例 ,缓解 2 2例 )患者及 10名正常对照Evi1基因的表达。结果 ① 10名正常人骨髓单个核细胞中无Evi1mRNA的表达 (阴性 ) ,HEL细胞系Evi1阳性。②AML患者Evi1基因总的阳性表达率为 18.1% (17/94) ,M5型未见表达 ,M1~M4各型的阳性率无差异 (P >0 .0 5)。AML初治、复发、完全缓解的Evi1基因阳性率分别为 2 6.5%、17.4%、4.5% ,各组间无明显差异 (P >0 0 5)。③AML初治患者中Evi1阳性表达者首次完全缓解 (CR1)后缓解持续时间短于Evi1阴性表达者 (P <0 0 1) ,早期病死率 (确诊后 3个月内死亡 )明显升高 (P <0 .0 1)。结论 Evi1基因在AML的发病中可能起一定作用 。
Objective To inves tigate the expression of Evi1 gene in acute myeloid Leukemia (AML).?Methods The expression of Evi1 mRNA was detected in HEL leukemia cell line, bone marrow mononuclear cells (MNC) from 94 AML patients (49 newly diagnosed, 23 relapsing, 22 in complete remission) and 10 healthy subjects, by semiquantita tive reverse transcriptase -polymerase chain reaction (RT-PCR).?Resu lts ① Evi1 mRNA, not expressed (negative) in 10 healthy subjects, was positive in HEL cell line. ② The positive rate of Evi1 mRNA expression in AML was 18.1% (17/94). The positive patients were of all AML subtypes, except M5 . The positive rate was 26.5% for the newly diagnosed, 17.4% for those in relaps e, and 4.5% for those in complete remission. There were no significant differen ces in positivity among AML subtypes or among the states of the AML ( P >0.05) . ③ The remission phase after CR1 was shorter in Evi1(+) patients than in the E vi1(-), and the early mortality rate was higher in the former than in the latter .?Conclusion Evi1 gene might play an important role in the pathogenesis of some myeloid malignancies. It may be taken as an index of p oor prognosis in AML.
出处
《徐州医学院学报》
CAS
2003年第6期535-538,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省教育厅自然科学基金部分资助(JW970 0 47)