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Evi1基因在急性髓系白血病中的表达及意义 被引量:1

Expression of Evi1 gene in acute myeloid leukemia and its sign ificance
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摘要 目的 探讨急性髓系白血病 (AML)中Evi1基因的表达及意义。方法 应用半定量RT -PCR方法检测了HEL白血病细胞系 (Evi1阳性细胞株 )、94例AML(初治 49例 ,复发 2 3例 ,缓解 2 2例 )患者及 10名正常对照Evi1基因的表达。结果 ① 10名正常人骨髓单个核细胞中无Evi1mRNA的表达 (阴性 ) ,HEL细胞系Evi1阳性。②AML患者Evi1基因总的阳性表达率为 18.1% (17/94) ,M5型未见表达 ,M1~M4各型的阳性率无差异 (P >0 .0 5)。AML初治、复发、完全缓解的Evi1基因阳性率分别为 2 6.5%、17.4%、4.5% ,各组间无明显差异 (P >0 0 5)。③AML初治患者中Evi1阳性表达者首次完全缓解 (CR1)后缓解持续时间短于Evi1阴性表达者 (P <0 0 1) ,早期病死率 (确诊后 3个月内死亡 )明显升高 (P <0 .0 1)。结论 Evi1基因在AML的发病中可能起一定作用 。 Objective To inves tigate the expression of Evi1 gene in acute myeloid Leukemia (AML).?Methods The expression of Evi1 mRNA was detected in HEL leukemia cell line, bone marrow mononuclear cells (MNC) from 94 AML patients (49 newly diagnosed, 23 relapsing, 22 in complete remission) and 10 healthy subjects, by semiquantita tive reverse transcriptase -polymerase chain reaction (RT-PCR).?Resu lts ① Evi1 mRNA, not expressed (negative) in 10 healthy subjects, was positive in HEL cell line. ② The positive rate of Evi1 mRNA expression in AML was 18.1% (17/94). The positive patients were of all AML subtypes, except M5 . The positive rate was 26.5% for the newly diagnosed, 17.4% for those in relaps e, and 4.5% for those in complete remission. There were no significant differen ces in positivity among AML subtypes or among the states of the AML ( P >0.05) . ③ The remission phase after CR1 was shorter in Evi1(+) patients than in the E vi1(-), and the early mortality rate was higher in the former than in the latter .?Conclusion Evi1 gene might play an important role in the pathogenesis of some myeloid malignancies. It may be taken as an index of p oor prognosis in AML.
出处 《徐州医学院学报》 CAS 2003年第6期535-538,共4页 Acta Academiae Medicinae Xuzhou
基金 江苏省教育厅自然科学基金部分资助(JW970 0 47)
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参考文献12

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同被引文献11

  • 1李春蕊,刘文励,孙汉英,周剑锋,黄亮,黄伟.髓系白血病中EVI1基因的表达及其临床意义[J].中国实用内科杂志,2006,26(6):820-822. 被引量:1
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  • 5Cuenco GM,Nucifora G,Ren R. Human AML1/MDS1/ EVI1 fusion protein induces an acute myelogenous leukemia (AML) in mice:a model for human AML[J].{H}Proceedings of the National Academy of Sciences(USA),2000,(04):1760-1765.
  • 6Louz D,van den Broek M,Verbakel S. Erythroid defects and increased retrovirally-induced tumor formation in Evi1 transgenic mice[J].{H}LEUKEMIA,2000,(11):1876-1884.
  • 7Goyama S,Kurokawa M. Pathogenetic significance of ecotropic viral integration site-1 in hematological malignancies[J].{H}CANCER SCIENCE,2009.990-995.
  • 8Langabeer SE,Rogers JR,Harrison G. EVI1 expression in acute myeloid leukaemia[J].{H}British Journal of Haematology,2001,(01):208-211.
  • 9Calmels B,Ferguson C,Laukkanen MO. Recurrent retroviral vector integration at the Mds1/Evi1 locus in nonhuman primate hematopoietic cells[J].{H}Blood,2005,(07):2530-2533.
  • 10Barjesteh S,Erpelinck C,van Putten WL. High EVI1 expression predict's poor survival in acute myeloid leukemia:a study of 319 de novo AML patients[J].{H}Blood,2003,(03):837-845.

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