摘要
目的 对中国不同鼠疫自然疫源地鼠疫菌染色体的分析和比较发现其近缘关系进而从分子水平进行分型。方法 用随机扩增多态性DNA(RAPD)技术。结果 第 1条引物将中国的鼠疫菌分为 11个类型 ,每个类型都有一定的条带规律和地理分布范围。第 2条引物将我国的鼠疫菌分为 5个类型。结论 随机扩增多态性DNA(RAPD)
ObjectiveTo analyze and compare the chrom osome of Yersinia pestis from various natural foci in China so that finding thei r relationships and typing in molecular level.MethodsRandom amplified polymorphic DNA (RAPD) was use d.ResultsYersinia pestis were divided into 11 types by t he first primer.Each type had a specific arrays pattern and geographic distribut ion. Yersinia pestis were divided into 5 types by the second primer.ConclusionsRAPD could be used to type Yersinia pestis in molecular level.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2003年第6期548-551,共4页
Chinese Jouranl of Endemiology
基金
中国科技部基础研究基金资助项目 ( 2 0 0 0 0 730 )
