恒温消解-流动注射氢化物原子吸收法测定血清中硒

Determination of the serum selenium by digestion in constant tempera ture-flow-injection hydride generation-atomic absorption spectrometry method

目的 建立快捷、易行、准确的血清硒测定方法。方法 利用氯酸在 13 0℃恒温下消化样品 ,以流动注射氢化物原子吸收法测定血清硒。结果 方法的线性范围为 0～ 10 μg/L ,检出限为 0 .48μg/L ,平均回收率为96.5% ,合并变异系数为 6.2 %。结论 测定方法具有灵敏度高、重现性好、结果准确、操作简单、一次测定样品量大等优点 。

ObjectiveTo establish an accurate,rapid,simp le method for determination of selenium in the serum.MethodsThe serum selenium digested in chlorin acid at constant 130℃ was determined by flow-injection hydride generation-atomic abso rption spectrometry method.ResultsThe linear range of the method was 0～10 μg/L, detection limit 0.48 μg/L, the mean recovery rate 96.5% and the incorporated c oefficient of variation 6.2 %. ConclusionsThe method is simple, sensitive, accurate, repeatable and a large quantity of samples can be determined at a time. It is su itable for survey and study in selenium-deficiency section and the clinical dia gnosis of the disease in relation to selenium-deficiency.