盐酸布比卡因、透明质酸酶对大鼠在体肌卫星细胞增殖的影响

EFFECTS OF BUPIVACAINE AND HYALURONIDASE ON THE PROLIFERATION OF MUSCLE SATELLI TE CELLS IN ADULT RATS IN VIVO

目的 :研究盐酸布比卡因和透明质酸酶对成年大鼠肌卫星细胞在体增殖的影响。方法 :免疫组化法 ,H .E染色法 ,光镜和电镜观察。结果 :①正常对照组和生理盐水组肌纤维完整 ,有少量Desmin阳性肌卫星细胞 ,面密度值为 0 .66%± 0 .57%和 2 .48%± 1.13 %。生理盐水组较正常对照组无显著差异 (P >0 .0 5)。②透明质酸酶组肌纤维完整 ,Desmin阳性肌卫星细胞数量增加 ,面密度值为 2 .52 %± 1.41% ,较生理盐水组和正常对照组无显著差异(P >0 .0 5)。③盐酸布比卡因组和盐酸布比卡因 +透明质酸酶混合液组均可见大量坏死和溶解的肌纤维 ,并伴有肌卫星细胞的激活、增殖 ,Desmin阳性肌卫星细胞显著增加 ,并有部分融合形成小肌管。面密度值分别为 19.0 1%± 4.74%和 2 2 .41%± 7.64% ,较生理盐水组显著增加 (P <0 .0 1)。结论 :局麻药盐酸布比卡因能引起在体肌卫星细胞的活化、增殖并形成肌管 。

Aim: To study the effects of Bupivacaine and hyaluronidase on the prolif eration of adult rat muscle satellite cells in vivo. Methods: Im munohistochemistry, hematoxylin and eosin staining, electron micrograph were use d.Re sults:①There are few rare desmin positive satellite cells lie in the myof ibers of control group and Sterile saline group which are still continual. MMD o f control and Sterile saline group is 0.66%±0.57% and 2.48%±1.13% respectively . Sterile saline group has no significant difference than that of the control( P> 0.05 ). ②The myofibers of hyaluronidase group are basically continual.Th e nu mber of desmin positive satellite cells are increased.MMD of Hyaluronidase is 2. 52%±1.41% which has no remarkable difference than that of the Sterile saline ( P> 0.05 ).③Plentiful necrosis and degeneration myofibers can been seen in Bup ivacaine group and Hyaluronidase+Bupivacaine group coinciding with the activatio n and proliferation of muscle satellite cells. The number of Desmin positive sat ellite cells are increased significantly and some of which have formed myotubes . MMD of Bupivacaine and Hyaluronidase+Bupivacaine is 19.01%±4.74% and 22.41%± 7.64% respectively which have significant change than that of Sterile saline(P <0.01). Conclusion:The local anaesthetic Bupivacaine can induc e the significant proliferation of myoblasts and the formation of myotubes in vivo. Hyaluronidase has no significant effect on the proliferation of satellit e cells in vivo under this experimental condition.