慢性强直电刺激右侧尾壳核诱导大鼠癫痫样电图和行为发作特征

CHARACTERISTICS OF ELECTROGRAPHIC AND BEHAVIORAL SEIZURES INDUCED BY CHRONIC TET ANIZATION OF THE RIGHT CAUDATE-PUTAMEN IN RATS

目的 :慢性强直电刺激右侧尾壳核 (CPu)诱导大鼠电图和行为癫痫点燃样现象 ,观察CPu或海马 (HPC)网络异常的靶向癫痫样行为表达特征。方法 :共用雄性SD大鼠 58只。强直电刺激 ( 60Hz ,0 .4～ 0 .6mA ,2s)大鼠右侧CPu或右侧前背HPC ,1time/d ,连续刺激 7～ 12d。结果 :①CPu电图节律性尖波样发放或HPC电图阵发性高幅失律。②CPu或HPC刺激组大鼠均可以出现原发性、继发性或点燃样湿狗样抖动 (wetdogshakes ,WEDS)、直立、洗面、好静、咀嚼和节律性点头等行为发作。③CPu刺激组大鼠原发性WEDS频率明显低于HPC刺激组大鼠( 2 .10± 0 .12和 2 .89± 0 .2 0times/min ,P <0 .0 1) ,继发性WEDS频率明显高于HPC刺激组大鼠 ( 1.2 3± 0 .11和0 .78± 0 .0 6times/min ,P <0 .0 1)。④CPu刺激组大鼠点燃样效应出现之前的行为静止天数较长。结论 :如同刺激HPC一样 ,慢性电刺激大鼠CPu可以出现类似的癫痫样行为发作。结果提示 :CPu功能异常有可能成为癫痫发作的起源病灶 ,与HPC类似 ,参与了颞叶癫痫电网络的重建 。

Aim:The electrographic and behavioral kindling effects were induced by c hronic tetanization of the right caudate-putamen (CPu) to study the target-beh avior expression involved in the CPu or hippocampus(HPC) network abnormalities. Methods: Experiments were performed on 58 SD rats. Tetanization (60Hz,0.4～0.6mA, 2s) was delivered into the CPu or the HPC, once a day, for 7～12 days. Animal behaviors were observed every day and depth electrographs wer e recorded at the beginning or at the end of the experiments. Results: Chronic tetanization of the CPu or of the HPC induced: ①Rhythmic sharp waves in the CPu and paroxysmal epileptiform events in the HPC electrographs. ② Primary behavioral seizures, secondary behavioral seizures, and kindling effects , including wet dog shakes(WEDS), rearing, face washing, immobility, chewing and head nodding. ③Lower rate of primary WEDS (P< 0.01 ), and higher rate of secondary WEDS (P<0.01) in the CPu-tetanized rats. ④Longer silent period of behavioral seizures before kindling appeared in the CPu-tetanized rats. Conclusion: Kindling effects in the CPu-tetanized rats resembles th ose in the HPC-tetanized rats. The CPu might participate in the origin of epile ptic focus and be involved in reestablishment of limbic epileptic networks, whic h may be responsible for the target-behavioral seizures.