原发性肝细胞癌患者癌细胞及癌旁组织中HBV-DNA及HBV抗原的定位研究

Detection of HBV-DNA and HBV antigens in cancer and surrounding tissues of patients with primary hepatocellular carcinoma

应用原位核酸杂交技术和免疫金银染色法分别检测42名原发性肝癌(PHC)患者肝癌及癌旁组织中的HBV-DNA 和 HB 抗原。结果:28例(66.7%)癌旁组织中 HBV-DNA 阳性,其中17例癌组织中也阳性.免疫组化结果显示 HBsAg 阳性者24例、HBcAg 阳性者6例,均见于癌旁组织。提示本组病例 HBV 感染是 PHC 的主要病因之一。本文部分 PHC 患者癌旁组织中仍有活跃的病毒复制,但癌组织中却未见表达相应抗原,推测 HBV 与机体长期作用后,部分病毒通过某种机制从免疫反应中逃逸并整合进入肝细胞基因组中,诱发恶变,可能是 PHC 的主要发病机制之一。

By adapting the in-situ hybridization and immunal gold-silver staining method,we detected HBV-DNA and HBV antigens in the cancer cells and tissues surrounding the cancer in 42 cases of primary hepatocellular carcinoma(PHC).The results showed that HBV-DNA was positive in 28 (66.7%),HBsAg positive in 24 and HBcAg positive in 6.Both HBsAg and HBcAg were seen in tis- sues surrounding the cancer,suggesting that HBV infection is the major cause of PHC in this group.Active replication of the virus was observered in the surrounding tissues in some PHC pa- tients,but existed no corresponding antigen in cancer cells,which helps deduce that with long action on the body,some viruses escape from immunoreaction through certain mechanism,get in- tegrated into the genome of liver cells,and thus induce malignant transformation,which might be the main pathogenesis of PHC.