百合脱毒及病毒检测技术研究进展

Advances of Research on Eradication and Detection of Viruses in Lily

百合是重要的观赏花卉 ,百合的繁殖主要通过无性繁殖方式进行 ,由于病害的侵染 ,常常造成植株体内的病毒积累 ,导致植株发生病毒病 ,最终严重降低百合植株的经济价值和观赏价值。因此 ,防止百合感染病毒病的应对策略是采用脱毒培养技术获得脱毒苗 ,并在各个生产环节进行严格的病毒检测。作者系统地介绍了常用的脱毒技术———茎尖培养、珠芽培养、化学处理和热处理 ,以及主要病毒检测方法———指示植物法、电镜技术、酶联免疫法和分子生物学技术。

Lily is an important ornamental flower.Since most of lilies are usually propagated vegetatively,which make viruses accumulate in plants.Therefore,virus diseases often occur in lily,and greatly decrease the economic and ornamental value of lily.The production of virus-free plants is an important approach to control virus diseases.The virus-free plants could be obtained through the tissues culture of eradicating viruses.However,strict virus detection is needed at each stage of production process.This paper systematically presented the basic eradicating viruses techniques-meristem culture in vitro,bulblet culture,chemotherapy and thermotherapy;and main detecting virus methods-indicator plants,electron microscope,enzyme linked immunosobent assay(ELISA),and techniques of molecular biology.