摘要
在已有的试管凝集法检验仔猪腹泻疫苗介导产生抗体效果的基础上,建立了用间接ELISA法检查K-88抗体活性的方法。用K-88抗原包板,羊抗兔酶标二抗检测经猪痢疫苗免疫后的兔血清所产生K-88抗体的情况。结果在一个月内抗体效价达到1∶40。与原有试管凝集试验结果比较,此法灵敏度较高,特异性较好,可快速、准确提供K-88抗体活性数据。
An indirect ELISA for detecting antibody to K-88was established by coating the mi croplate with K-88antigen.The rabbit antibody titer reached1∶40in one month after immunized with diarrhea of piglets.The result showed that the method established here is a rapid and precise assay with high sensitivity and specificity compared with TAT test for detecting the antibody to K-88levels.
出处
《生物技术通讯》
CAS
2003年第5期401-402,共2页
Letters in Biotechnology
