一种高效转导蛋白质入哺乳细胞的方法——HIV TAT介导法

An efficient way of HIV mediated protein transduction into mammalian cells

HIVTAT介导蛋白质入哺乳细胞的方法1988年就有文献报道,但近年才日趋成熟,且广泛应用于分子生物学研究。传统的调控哺乳细胞的途径有转导目的蛋白的表达型载体、微注射目的蛋白等。与之相比,TAT介导法有操作简单、迅速、应用广泛、可控性强等优点。

Although HIV TAT mediated protein transduction is originally reported in1988,it has been improved greatly recently.In many fields of molecular biology,mammalian cells have been successfully manipulated by classic methods such as transfection of expression vectors?microinjection.Compared with them,the TAT mediated protein transduction has many virtues such as simple and rapid procedure,extensive applicability,strong regulability.