雌二醇β-estradiol促进鼠纤维肝及氧化压力下培养的肝细胞中GPx的活性

β-estradiol enhances GPx activity in rat fibrotic liver and in cultured rat hepatocytes undergoing oxidative stress

目的 :研究雌二醇β-estradiol与肝细胞中抗氧化酶—谷胱甘肽过氧化物酶 (Glutathione peroxidase,GPx)活性之间的关系。方法 :用 dimethylnitrosamine(DMN)诱导的鼠肝纤维化模型及 ferric nitrilotriacetate solution(Fe NTA)导致分离培养的肝细胞经历氧化压力 ,在雌二醇存在与不存在的条件下检测 GPx活性。结果 :在活体中及在体外细胞培养中表明雌二醇β-estradiol促进鼠纤维肝及氧化压力下培养的肝细胞中 GPx的活性。在活体中 0 .5 mg/ kg· d的 estradiol valerate剂量使 GPx的活性增强为对照组的 1.73倍 (q=6 .5 9,P<0 .0 1) ;在体外细胞培养中 10 - 6mol/ L的β-estradiol剂量 GPx的活性增强为对照组的 2 .6 8倍 (q=10 .5 9,P<0 .0 1)。结论 :雌激素 β-estradiol抑制肝纤维化的机理之一是其促进肝细胞中的抗氧化酶— GPx的活性。

Objective:To study the relation of β-estradiol and the Glutathione peroxidase (GPx) activity in rat fibrotic liver and in cultured rat hepatocytes undergoing oxidative stress.Methods:Hepatic fibrosis was induced by dimethylnitrosamine(DMN) with or without estradiol valeratel for 2 weeks and hepatocytes were cultured with ferric nitrilotriacetate solution(FeNTA),with or without β-estradiol for 24 hours, then the GPx activity in rat liver and in cultured rat hepatocytes were analysed.Result:β-estradiol enhances the GPx activity in rat fibrotic liver and in cultured rat hepatocytes undergoing oxidative stress. The GPx activity in rat fibrotic liver with 0.5 mg·kg -1/d of estradiol valerate was 1.73 times(q=6.59,P<0.01) that of without estradiol valerate.The GPx activity in cultured rat hepatocytes undergoing oxidative stress with 10 -6 mol/L of β-estradiol was 2.68 times(q=10.59,P<0.01) that of without β-estradiol. Conclusion:β-estradiol enhances the GPx activity in rat fibrotic liver and in cultured rat hepatocytes undergoing oxidative stress.