川芎嗪逆转肺癌细胞株多药耐药性的研究

Study on Tetramethylphylpyrazine Reversing Multi - drug Resistance of Lung Cancer Cell Line

目的 探讨中药川芎嗪(TMP)逆转肺腺癌SPCA-1细胞株耐药性的作用。方法 人肺腺癌SPCA-1细胞株,以阿霉素诱导耐药至终浓度为0.4μg/ml,MTT比色法测定TMP对耐药细胞的耐药性逆转作用。结果 100mg/L和320mg/L TMP对细胞的抑制率均小于5％,两者无显著差异。100mg/L TMP加丝裂霉素(MMC)组与相应浓度MMC组比较,细胞抑制率有显著性差异(P<0.05)。加入320mg/L TMP的阿霉素(ADM)、长春地辛(VDS)、MMC组与相应浓度各药物组比较,其细胞抑制率均有显著性差异(P<0.05),其中1PPC VDS+TMP、1PPC MMC+TMP组与相应药物组比较,差异非常显著(P<0.01)。顺铂(C-DDP)加TMP组与相应浓度C-DDP组比较,细胞抑制率均无显著性差异(P>0.05)。结论 无明显细胞毒浓度的TMP可显著逆转SPCA-1人肺腺癌细胞株对ADM、VDS、MMC的耐药性,但对CDDP介导的耐药无影响。

Objective To investigate the action of tetramethylphylpyrazine (TMP) reversing drug-resistance in SPCA -Ⅰ lung adenocarcinoma cells. Methods SPCA -Ⅰ lung cancer cells were induced by adriamycin which the last concentration was 0.4 g/ml. The sensitivity of chemical drugs on SPCA- Ⅰ/ADM cells was detected by MTT colorimetric assay. Results When the concentration of TMP was 100mg/Land 320mg/L, Both the inhibition rate were less than 5% . When the concentration of TMP was 100mg/L, there was significant difference of inhibition rate in MMC + TMP group and MMC control group(P < 0. 05). When the concentration of TMP is 320nig/L, it was also significant difference of inhibition rate in ADM +TMP, VDS + TMP, MMC + TMP groups and their control groups (P < 0. 05 = Furthermore, there was very significant difference in VDS + TMP, MMC + TMP groups and their control groups (P <0. 01= which the concentration of VDS and MMC was 1PPC. But, there was no significant difference in CDDP + TMP groups and its control groups(P > 0. 05). Conclusion The drug - resistance to ADM, VDS and MMC in SPCA -Ⅰ/ADM cells can be reversed by non - cell toxic concentration of TMP, the drug - resistance to CDDP is not affected.