p53p21双基因转染对肝癌细胞生长的抑制作用研究

Inhibition effect of co-expression of human wild type p53 and p21 genes on hepatocellular carcinoma

目的 研究 p5 3和 p2 1双基因对肝癌细胞的联合基因治疗效果。 方法 通过磷酸钙 -DNA共沉淀法将p5 3和 p2 1双基因真核共表达载体及单基因表达载体引入肝癌细胞SMMC 772 1,用直接镜检 ,DNAladder检测法和四甲基偶氮唑 (MTT)法等研究细胞生长情况。结果 转染外源 p5 3p2 1双基因的肝癌细胞SMMC 772 1与未转染及单基因转染的肝癌细胞SMMC 772 1相比 ,其增殖速度显著下降。结论 p5 3和p2 1双基因对肝癌细胞的联合基因治疗效果比较明显 ,对肝癌基因治疗的进一步研究具有指导意义。

Objective Research the effect of co-gene therapy of human wild type p53 and p21 genes on hepatocellular carcinoma. Methods Construct the eucaryon vector co-expressing human wild type p53 and p21 genes. The vector with wild type p53 and p21 genes was introduced into cancer cells through methods of calcium phosphate-DNA co-deposition. Direct microscopy, DNA ladder and monotetrazolium(MTT) test was performed to measure the effect. Results It was showed that most cells were transfected and the growth rate was apparently arrested while apoptosis was observed. Conclusion The results suggest that the external wild type p53 and p21 genes transferred can induce apoptosis in hepatocellular carcinoma. The effect of co-gene therapy of p53 and p21 on hepatocellular carcinoma is obvious.