鲜壁虎提取物抑制C6胶质瘤细胞增殖和诱导凋亡的研究

Effects of natural extraction of gecko in inducing apoptosis and antiproliferation of C6 glioma cells

目的 研究鲜壁虎提取液对C6胶质瘤细胞的致凋亡作用及其方式。方法 用不同浓度的鲜壁虎液处理处理C6胶质瘤细胞 ,用MTT法观察增殖抑制 ,用透射电镜观察用药后细胞形态的变化 ,用琼脂糖凝胶电泳观察用药后细胞DNA断裂情况 ,用流式细胞术定量观察C6细胞凋亡情况。结果 经5 0mg/L鲜壁虎液处理的C6胶质瘤细胞 ,增殖能力降低 ;5mg/L ,30mg/L ,5 0mg/L鲜壁虎液均可以使C6细胞显示明显凋亡征象 ,有DNA断裂现象 ,并有浓度和时间依赖性。结论 鲜壁虎液在体外能诱导C6胶质瘤细胞凋亡 ,抑制细胞增殖 ,是一种具有抗肿瘤作用的天然药物。

Objective To study the mode of the action of the natural extraction of gecko on C6 glioma cells.Methods C6 glioma cells treated by various concentrations of natural extraction of gecko were assessed.Antiprolifeation was obtained by MTT, DNA gel electrophoresis and cell morphology study were used to observe the evidence of apoptosis.Quantitative analysis of apoptosis was done by flow cytometry.Results The cells treated by 50mg/L natural extraction of gecko showed significant antiproliferation, 5mg/L, 30mg/L, 50mg/L natural extraction of gecko can induce C6 glioma cells apoptosis, DNA gel electrophoresis showed DNA rupture, implying C6 glioma cells apoptotic death to be dose and time dependent.Conclusion The natural extraction of gecko can induce C6 glioma cells apoptosis and decrease cell proliferation, suggesting that natural extraction of gecko might be a potential anticancer drugs.