大鼠脊髓损伤后血红素氧化酶-1的表达

Expressions of heme oxygenase-1 in the traumatized rat spinal cord

目的 研究血红素氧化酶 - 1(HO - 1)及其mRNA在脊髓损伤后表达变化。 方法用免疫组织化学技术和原位杂交技术 ,观察正常大鼠、脊髓损伤后 1d和 3d大鼠脊髓HO - 1及其mRNA的分布和含量变化。 结果 正常大鼠脊髓HO - 1表达较少 ,主要在神经元 ;而损伤后表达明显增加 ,主要是小胶质细胞和星形胶质细胞 ,并围绕在血肿周围。损伤后 1d蛋白的表达开始增加 ,阳性细胞的灰度和面积分别为 (14 8.2 6± 11.39)和 (90 .5 0± 8.70 )× 10 3 μm2 ;损伤后 3d表达强度进一步升高 ,阳性细胞的灰度和面积分别为 (12 8.0 3± 12 .5 9)和 (112 .99± 10 .0 1)× 10 3 μm2 。损伤后 1d ,HO - 1mRNA表达阳性细胞的灰度和面积为 (10 6 .0 2± 9.10 )和 (70 .0 5± 9.2 6 )×10 3 μm2 ;3d时为 (85 .82± 9.0 7)和 (87.37± 10 .95 )× 10 3 μm2 ,与对照组相比差异有非常显著性意义(P <0 .0 1)。 结论 大鼠脊髓损伤后 ,HO - 1表达显著增加 ,有一定的保护作用。

Objective To observe the expression changes of heme oxygenase-1(HO-1) and its mRNA after spinal cord injury (SCI). Methods SD rats were randomly divided into normal (n=5), control (n=5) and experimental group (n=10). The rats were given no operation in the normal group, laminectomy only in the control and both laminectomy and traumatic injury in the experimental group. The SCI animal models were made by using a modified Allen's weight-drop device (50 g.cm) on T 12 . Rats were sacrificed at 1 and 3 days after injury. The distribution and content changes of HO-1 and its mRNA in spinal cord segments were observed by means of immunohistochemistry and in situ hybridization. Results Expression of HO-1 was mainly in neurons in the normal spinal cord. After injury, HO-1 expressed primarily in astrocytes and microglia and the distribution of positive cells closely correlated with intraparenchymal hemorrhage. Protein began to increase at the first day after injury. The expression intensity and the area of positive cells were (148.26±11.39) and (90.50±8.70)×10 3 μm 2 respectively. At the third day, protein expressed more significantly with expression intensity of (128.03± 12.59) and area of (112.99±10.01)×10 3 μm 2. The expression intensity and area of positive cells expressed by mRNA were (106.02±9.10) and (70.05±9.26)×10 3 μm 2 respectively at the first day after injury, (85.82±9.07) and (87.37±10.95)×10 3 μm 2 respectively at the third day after injury. There was significant difference between experimental and control groups ( P <0.01). Conclusions After SCI, significant expression increase of HO-1 may have certain protective effect.