养肝柔肝法逆转豚鼠成石胆汁致石性的实验研究

Experimental study on effects of herbs for nourishing and smoothing the liver in reversing bile lithogenicity of guinea pig

目的 探讨养肝柔肝中药养肝利胆颗粒剂逆转成石胆汁致石性的机制。方法 将 6 0只豚鼠随机分为正常组、模型组、治疗组 ,每组 2 0只。分别喂食正常饲料、致石饲料、致石饲料加养肝利胆颗粒 ,4周后处死取材 ,检测胆囊成石率、胆汁总胆汁酸 (TBA)、胆汁总胆红素 (TBIL)、胆汁结合胆红素 (CB)、胆汁非结合胆红素 (UCB)、胆汁钙离子 ,并进行胆汁胆红素组分HPLC分析。结果 (1)正常组成石率为 5 % ,模型组为 81.2 5 % ,治疗组为 31.2 5 % (P <0 .0 5 )。 (2 )模型组胆囊胆汁中TBIL、CB、UCB及Ca2 + 均高于治疗组及正常组 ,TBA低于正常组 ,治疗组TBA低于正常组 ,Ca2 + 高于正常组 (P <0 .0 5 )。(3)模型组单结合胆红素 (MCB)值高于治疗组及正常组 ,双结合胆红素 (DCB)低于治疗组及正常组 (P <0 .0 1) ,治疗组DCB值低于正常组 ,各组UCB的完整峰面积 (IPA)值无明显区别。 (4)模型组的MCB百分比和UCB百分比高于治疗组及正常组 ,DCB百分比低于治疗组及正常组 (P <0 .0 5 ) ,治疗组的MCB百分比也高于正常组 ,同时DCB低于正常组。结论 养肝柔肝中药能显著降低豚鼠胆囊色素性结石的成石率 。

Objective To further probe the mechanisms of herbs for nourishing and smoothing the liver in reversing bile lithogenicity of guinea pig. Methods Sixty guinea pigs were divided randomly into control group (fed with normal diet,n=20), model group (fed with lithogenic diet, n=20) and treatment group (fed with lithogenic diet plus herbal medicine,n=20). After four-week feeding, the animals were sacrificed and sampled, the rates of gallstone formation in each group were estimated, and the total bile acid (TBA), total bilirubin(TBIL), conjugated bilirubin(CB), unconjugated bilirubin(UCB), and calcium ion in the bile were determined, and the different bilirubins were analyzed by HPLC. Results (1) The rate of gallstone formation was 5% in normal group, 81.25% in model group and 31.25% in treatment group(P<0.05). (2) The bile TBIL, CB, UCB and Ca 2+ were higher and the bile TBA was lower significantly in model group than that in the other two groups(P<0 05). (3) HPLC analysis revealed that MCB was higher and DCB was lower significantly in model group(P<0.01), and there were no significant differences of UCB and IPA among the three groups. (4) The percentages of MCB and UCB were much higher and the percentage of DCB was remarkably lower in model group(P<0.05). Conclusion Herbs for nourishing and smoothing the liver can significantly reduce the rate of gallstone formation and has effect of reversing lithogenicity of bile in guinea pigs fed with lithogenic diet.