阿魏酸对培养视网膜神经细胞增殖活性的影响

Effect of ferulic acid on the proliferation of nerve cells of retinas in vitro

目的 研究阿魏酸促进视网膜神经细胞活性的可能机制 ,为治疗退行性视网膜病变提供依据。方法 以 7个月人胚胎视网膜、新生小牛视网膜和生后 4个月小鼠视网膜为研究对象 ,以细胞培养、MTT法和氚标记胸苷掺入法等为研究手段 ,检测阿魏酸、脑源性神经营养因子 (BDNF)和葛根素作用 72h后 ,视网膜神经细胞增殖和DNA合成变化。结果 阿魏酸对 3种视网膜神经细胞均具有促增殖作用 ,细胞活性强度呈现剂量依赖性 ,对于小鼠视网膜神经细胞 ,阿魏酸在 15 6～10 0 0 0mg/L内均具有促细胞增殖作用 ;对于新生小牛和 7个月人胚胎视网膜神经细胞 ,阿魏酸在12 5 0～ 10 0 0 0mg/L内具有促细胞增殖作用。对 7个月人胚胎视网膜神经细胞 ,阿魏酸在 6 2 5～10 0 0 0mg/L内具有促DNA合成作用 ,最佳效应浓度为 5 0 0 0mg/L ;BDNF在 12 5～ 5 0 0 μg/L内具有促细胞增殖作用 ,最佳效应浓度为 5 0 0 μg/L ;葛根素未见明显促增殖效应。 结论 阿魏酸对3种视网膜神经细胞的促增殖作用明显强于BDNF。阿魏酸能有效提高视网膜神经细胞活性 ,有望成为一种新的防治退行性视网膜病变的有效成分。

Objective To explore the possible mechanism of ferulic acid(FA) on the proliferation of nerve cells of retinas in vitro and provide the principal for prevention and treatment of some degenerative retinal diseases. Methods Retinal cells suspension from 7 months human embryo, neonatal calf , and 4 months postnatal mice were treated with FA, brain-derived neurotrophic factor(BDNF) and kakonein were used as palcebo. 72 hours after incubation with BDNT, MTT and 3H-thymidine ( 3H-TdR) incorporation were used to evaluate cell proliferation and DNA synthesis. Results The proliferation of three retinas cell lines was stimulated by the treatment of FA, it was shown that the effect of FA on cell viability was dose-dependent , and the highest response was seen in mice retinal cell. It has been found that to the adult mice group, FA within 15.6 to 1 000.0 mg/L was shown promotion for nerve cell proliferation, but only FA with 125.0-1 000.0 mg/L to that of 7 months human embryo, neonatal calf. To the 7 months human embryo group, FA with 62.5-1 000.0 mg/L was shown the promotion for DNA synthesis, moreover, 500.0 mg/L was most suitable for cell proliferation and DNA synthesis. BDNF with 12.5-50.0 μg/L was also shown promotion for nerve cell proliferation, moreover the 50.0 μg/L was the most suitable, but kakonein was not shown obvious promotional effect. Conclusions These results suggested that FA was better for nerve cell proliferation than that of BDNF, and would probably become one of new agents for prevention and treatment of some degenerative retinal diseases.