MAD2和p55CDC在前列腺癌组织中的表达及其与前列腺癌分级的相关性

Expressions of MAD2 and p55CDC in prostate cancer and their correlations with the prostate cancer grading

目的 :研究有丝分裂检查点调控蛋白MAD2和 p5 5CDC在前列腺增生组织和前列腺癌组织的表达情况 ,了解此两种蛋白的表达水平是否同前列腺癌的分级存在相关性。方法 :采用免疫组化的方法 ,对 4 6例前列腺增生组织和 6 5例前列腺癌组织的MAD2和 p5 5CDC蛋白表达水平进行检测 ,并对前列腺增生组织和前列腺癌组织以及不同分级的前列腺癌组织的MAD2和 p5 5CDC蛋白表达水平进行统计学比较和分析。 结果 :发现前列腺癌组织的MAD2和 p5 5CDC蛋白表达水平与前列腺增生组织相比明显下降 ,而且随前列腺癌分级的升高 ,MAD2和p5 5CDC蛋白表达水平呈进行性下降。 结论 :前列腺癌组织常存在MAD2和 p5 5CDC蛋白表达的缺失 ,而这两种蛋白的表达水平与Gleason分级存在密切相关性。发现了有丝分裂检查点调控蛋白MAD2和 p5

Objective:To investigate if downregulation of two mitotic checkpoint proteins, MAD2 and p55CDC, is a frequent event in human prostate cancer and whether decreased expression of these two proteins are associated with progression of prostate cancer. Methods: Using immunohistochemistry technique, the expressions of MAD2 and p55CDC proteins are examined in 46 benign prostate hyperplasia (BPH) and 65 prostate cancer tissues. Differential expressions were compared first between BPH and prostate cancer specimens, and then among prostate cancer samples with different Gleason grades. Results: We found that down regulation of MAD2 and p55CDC expressions was significant in prostate cancer (96% and 83%, respectively) compared to BPH (19.5% and 4.3%, respectively) ( P <0.001). In addition, decreased expression levels of these two proteins were associated with increased Gleason grade. Conclusion: Our results indicate that loss of MAD2 and p55CDC protein expression is a frequent event in prostate carcinoma and the decreased expressions of these two proteins are associated with increased Gleason grade. Our results provide first in vivo evidence to support the hypothesis that down regulation of certain mitotic checkpoint proteins plays an important part in human tumourigenesis.