整合素α6β4和实验性变态反应性神经炎

Integrin α6 β4 and experimental allergic neuritis

目的 :研究整合素α6和 β4在实验性变态反应性神经炎 (experimentalallergicneuritis,EAN)中的表达变化 ,探讨其表达变化与病变过程中髓鞘损伤和修复的关系。方法 :建立Lewis大鼠EAN动物模型 ,分别在急性期和恢复期的模型鼠坐骨神经 ,用免疫组化的方法观察α6、β4及层黏连蛋白的蛋白表达状况 ;用原位杂交的方法检测α6 β4的mRNA的表达状况。 结果 :免疫组化结果表明 ,β4在急性期表达减少 (P <0 .0 5 ) ,恢复期表达无明显变化(P =0 6 84 0 )。α6和层黏连蛋白在病变急性期和恢复期与对照组比较变化均不明显 (α6P =0 .0 75 1 ,层黏连蛋白P=0 .2 0 4 7) ,原位杂交也得出相似结论 (急性期 β4P <0 .0 5 ,恢复期β4P =0 .82 3;α6P =0 .81 )。 结论 :炎症损伤影响了施万细胞 (Schwanncell,SC)整合素的表达 ,其表达模式与周围神经的胚胎发育过程中α6 ,β1及β4的表达变化相似 ,推测 β4与髓鞘再形成的关系更加密切。α6和 β4表达变化与髓鞘的损伤和修复过程密切相关。

Objective: To explore the relationship between integrins α6,β4 and the process of the injury and repair of the myelin, their expressions in experimental allergic neuritis were analyzed. Methods: Experimental allergic neuritis (EAN) models were induced in Lewis rats. The expressions of integrins α6 and β4 and their receptor laminin, both in the acute phase and recovery phase of EANs, were analyzed by in situ hybridization and immunohistochemistry. Results: The expression of β4 decreased in the acute phase( P <0.05) and showed no significant changes in the recovery phase( P =0 6840). There were no significant changes of the expression of α6 and laminin both in the acute phase and recovery phase of EANs by immnohistochemistry (α6 P = 0.0751;laminin P =0.2047). The similar results were obtained by in situ hybridization (β4 in acute phase P <0.05;β4 in recovery phase P =0.823;α6 P = 0.81) . Conclusion: The expressions of integrins of Schwann cells were affected by inflammation injury, and their patterns of expression were similar to those of integrins α6, β1 and β4 during embryogenesis. It is inferred that integrin β4 and the reformation of myelin have a more imitate relationship. The expression changes of α6 and β4 correlate with the injury and repair of myelin during EAN.