低剂量长期饲喂La(NO_3)_3后La在大鼠骨中的蓄积及骨微结构的改变

La accumulation and microstructure change of leg bones of rats fed with La(NO_3)_3 in low dosage for a long term

目的 :研究大鼠低剂量长时间摄入稀土元素后稀土在其骨中蓄积及骨微结构的改变。方法 :以 2mg·kg-1 ·d-1 La(NO3 ) 3 灌胃饲养大鼠 6个月后 ,用ICP MS及分光光度法分别测定其腿骨中La及Ca、P含量 ,用电镜及X 射线粉末衍射观测骨微结构的改变。结果 :试验组鼠腿骨中La和P含量增加 ,Ca含量基本不变 ,Ca/P比值下降 ,骨小梁数目减少、变细 ,甚至断裂 ,骨小梁间空腔增大 ,但骨结晶度增加。结论 :大鼠长期摄入低剂量La(NO3 ) 3 可导致La在骨中蓄积 。

Objective: To study La accumulation and microstructure change of leg bones of rats fed with La(NO 3) 3 in low dosage for a long term. Methods: After the rats were fed by La(NO 3) 3 in dosage of 2 mg·kg -1 ·d -1 for 6 months, the contents of La and Ca,P in the leg bones were determined by ICP MS and spectrophotometry; the microstructure changes of the leg bones were investigated by electron microscopy and X ray powder diffraction. Results: In the leg bones of tested rats, the contents of La and P increased greatly, and those of Ca did not change obviously, so that Ca/P ratio values decreased in comparison with the control group. Conclusion: La was accumulatied in the rat leg bones and the change of bone microstructure induced after the rats were fed with La(NO 3) 3 in low dosage for a long term.