银杏根系甲醇溶提物对离体培养条件下丛枝菌根真菌生长发育的研究

Effect of methanol eluates from Ginkgo biloba root on growth and development of arbuscular mycorrhizal fungi cultured in vitro

银杏根系甲醇溶提物对离体培养条件下丛枝菌根真菌生长发育的试验结果表明 ,银杏根系甲醇溶提物对离体条件下丛枝菌根真菌 (Glomusmosseae,Gigasporamargarita)生长发育有明显的促进作用 ,能显著提高孢子萌发率 ,增加菌丝生长长度 .与对照相比 ,浓度为 2 0 %～ 10 0 %的甲醇溶提物均可显著促进丛枝菌根真菌的生长发育 ,80 %甲醇溶提物效果最明显 .银杏根系甲醇溶提物中含丰富的黄酮类化合物 ,溶提物中黄酮类物质的含量与甲醇洗脱剂的浓度有关 ,当浓度为 80 %时 ,黄酮含量最高 .对银杏根系甲醇溶提物中黄酮类化合物含量与丛枝菌根真菌生长发育相关性分析发现 ,溶提物中黄酮类化合物含量与离体条件下丛枝菌根真菌孢子的生长发育表现为极显著的正相关关系 .溶提物中黄酮含量越高 。

The effect of methanol eluates from Ginkgo biloba root on growth and development of arbuscular mycorrhizal fungi was studied under`cultured in vitro . The study indicated that the methanol eluates from Ginkgo biloba root could significantly stimulate the spore germination and hyphal growth of arbuscular mycorrhizal fungi ( Glomus mosseae,Gigaspora margarita ),and the 80% methanol eluates had the most significant effect. There were rich flavonoids in the methanol eluates,and the highest flavonoids content was in the 80% methanol eluates. The mathematical analysis showed that there was a significantly positive correlation between the flavonoids content in the methanol eluates and the growth and development of arbuscular mycorrhizal fungi cultured in vitro .