载脂蛋白E基因多态性与高血压肾损害及肾实质性高血压

Apolipoprotein E gene polymorphism with hypertensive renal injury and renal substantial hypertension

目的 探讨载脂蛋白E(ApoE)基因多态性与高血压 (EH)肾损害及肾实质性高血压 (RH)的关系。方法 采用PCR技术 ,对黑龙江地区 4 8例高血压 -肾功能正常病人 (EH -NRF)、4 6例高血压 -肾功能衰竭病人 (EH -CRF)和 5 2例肾实质性高血压 -肾功能正常病人 (RH -NRF)、5 6例肾实质性高血压 -肾功能衰竭病人 (RH -CRF)及 5 0例健康对照者 (NC)的ApoE基因多态性进行检测。结果 共检测出 3种基因型 ,纯合子缺失型 (DD)、纯合子插入型 (II)、杂合子插入 /缺失型 (ID)。EH -NRF组与NC组比较D等位基因及DD基因型差异无显著意义 (P >0 0 5 )。EH -CRF、RH -NRF、RH -CRF组D等位基因及DD基因型频率明显高于EH -NRF、NC组 ,差异有显著意义 (P <0 0 5 ,P <0 0 0 1)。结论 ApoE基因I/D多态性与EH发生无关 ,而与EH并发症呈明显相关 ,可能参与肾损害过程。D等位基因可能是黑龙江地区汉族人肾损害的遗传危险因素 ,但不能用ApoE基因多态性来解释EH ,说明高血压并发尿毒症与尿毒症并发高血压存在不同的遗传基础。

ObjectiveTo explore the relationship of the polymorphism of apolipoprotein E gene(ApoE) to hypertensive renal injury and renal substantial hypertension.MethodsBased on PRC technology,we tested the polymorphism of apolipoprotein E gene in 48 patients with hypertension and normal renal function(EH-NRF), 46 with hypertension hypertension and renal failure (EH-CRF), 52 with renal substantial hypertension and normal renal function (RH-NRF), 56 with renal substantial and renal failure (RH-CRF) and 50 normal controls(NC)in Heilongjiang.ResultsThree gene types (the DD,the II and the ID) were detected.D allele and DD which were in EH-NRF and NC had no obvious difference (P>0.05). The frequency of D allele and DD was higher in EH-CRF, RH-NRF, RH-CRF than that in EH-NRF, NC, and the difference was significant (P<0.05,P<0.001).ConclusionsThe I/D polymorphism of ApoE gene has no relationship with EH,but was obviously concerned with the complications of EH,which may play a role in renal injury.D allele may be the genetic risk of renal injury in Han in Heilongjiang.The polymorphism of ACE gene can not account for hypertension,which suggests there exist different genetic elements between hypertension with uremia and uremia with hypertension.