带有全息滤光片的激光共焦系统用于基因芯片的检测

A laser confocal scanning system with holographic filters used for genechip detection

提出了一个激光共焦扫描系统 ,对其应用于基因芯片的检测进行了实验研究。系统中用本实验室研制的全息滤光片代替通常所用的昂贵的窄带干涉滤光片和二色镜 ,检测得到的信号强度是背景信号的数十倍 ,是背景信号波动的数百倍 ,灵敏度达 2 .0× 10 - 2 2 mol/μm2 。通过扫描采样和图像重建 ,能给出荧光强度的二维图像信息 ,清晰地得出了芯片上的阳性导向点及一些阳性点。本文研究的结果表明 ,将全息滤光片应用于共焦系统对低密度芯片进行检测 。

A laser confocal scanning system equipped with holographic notch filters used for simple genechip detection is presented.The volume holographic notch filters were fabricated in our lab.to substitute for the expensive narrow-band interference filter and the dichroic filter in the conventional confocal detection system.The detection experiment was carried out on the gene-chips hybridized with cy5-labeled targets and the system sensitivity was determined by static measurement.The system has the sensitivity of 2.0×10 22 mol/μm 2 and can detect some positive spots on the practical genechips.The experimental results indicate that the confocal system can be used for the detection of some low-density genechips.