大獭蛤的胚胎、幼虫及稚贝的形态发育

The Morphogenesis of the Lutraria maxima's Embryo,Larvae and Spat

在广西海洋研究所海滨公园育苗场进行大獭蛤 (L utraria maxima Jonas)的胚胎发育观察及人工育苗实验。实验亲贝取自北海市侨港码头。通过人工授精获取受精卵。取部分受精卵在实验室用显微镜进行胚胎发育观察及拍照 ,在囊胚期之前每隔 10 m in取样 1次 ,囊胚期之后每个半小时取样 1次 ,胚胎发育观察至 D形幼虫期。大部分受精卵在育苗池中进行人工育苗实验 ,育苗期间每天取样 1次 ,在显微镜下进行观察、测量及拍照 ,并记录贝苗大小及发育期情况。实验期间水温变化为 2 5 .0～ 2 8.7℃ ,盐度为 2 9.3‰～ 31.9‰。根据观察记录结果 ,得出大獭蛤胚胎发育及贝苗生长发育图谱 ,并描述了大獭蛤的胚胎发育及其幼虫。

The trial was conducted to observe the embryogenesis of the eggs of Lutraria maxima Jonas and to develop its artificial hatchery technique The broodstocks used in the trial were wild mature clams collected from the sea and eggs were fertilized with artificial fertilization Embryonic development of the eggs from fertilized eggs to D larvae was observed under binocular microscope and photos were taken to record the development Samples were taken in an interval of ten minutes before blastula stage and in an interval of thirty minutes after blastula stage Artificial hatchery of the eggs was conducted in concrete ponds Samples were taken every day to observe the development and growth of the eggs, larvae, and spats Photos were also taken to record the metamorphasis During the trial,water temperature varied between 25 0～28 7℃, and salinity between 29 3‰～31 9‰ In this paper,detailed description and photo record on the embryogenesis and morphology of the eggs,larvae,and spats were presented