应用RAPD技术初步分析无裂栉江珧四种类型的基因组DNA

Preliminary RAPD Analysis of Four Groups in Atrina pectinata Linaeus

利用 3个随机引物 (S4 5 3,S4 5 4 ,S4 6 3)对无裂栉江珧 (Atrina pectinata L inaeus)的 4种类型 :青口、黄口、沙螺、棘螺基因组 DNA进行 RAPD分析 ,并对 RAPD的实验条件行了优化研究。10～ 2 0 ng的高纯度 DNA用作模板 ,退火温度为 39℃时 ,RAPD扩增的效率较高 ,扩增的带纹清晰可辨。3个引物中 ,引物 S4 5 3和 S4 6 3获得了清晰的 RAPD扩增结果 ,引物 S4 5 4未获得有效扩增 ,共扩增出 8条清晰的 DNA片段。8条清晰的类群特异或类群共享的 DNA片段 ,能将沙螺、棘螺、油螺 (青口与黄口的合称 )区分开来 ,尚未发现能将青口与黄口分开的

By RAPD analysis of four groups in Atrina pectinata Linaeus as green pen shell、yellow pen shell、thorny pen shell and scabrous pen shell,effective amplification was observed in the action of S453 or S463 As a result,some polymorphic DNA fragments of individual were revealed,8 clear group specific or group shared DNA fragments were selected for characteristic bands to preliminarily distinguish the three groups thorny pen shell,scabrous pen shell and oilily pen shell (a general designation of green pen shell and yellow pen shell).No characteristic band can be used to distinguish green pen shell from yellow pen shell