荧光定量PCR方法检测母乳中人类巨细胞病毒

Determination of Human Cytomegalovirus in Breast Milk With Fluorescent Quantity PCR

目的 :比较荧光定量PCR(FQ PCR)方法与定性PCR方法的敏感性 ,同时通过应用FQ PCR方法了解HCMV感染患儿母乳中HCMV感染情况。方法 :用FQ PCR方法与普通定性PCR方法共同检测 94份尿液中HCMVDNA ,将两种方法进行比较。用FQ PCR方法检测 76例HCMV感染患儿母乳中HCMVDNA。结果 :FQ PCR方法检测阳性率为 6 0 .6 % ,定性PCR方法检测阳性率 33.3% ,FQ PCR方法优于定性PCR方法。应用FQ PCR方法检测HCMV感染患儿母乳的HCMV阳性率为 72 .37%。结论 :FQ PCR方法优于PCR方法 ,应用FQ PCR检测母乳中HCMV是一种方便、快捷的方法。HCMV感染患儿母乳HCMV阳性率高 。

Objective: By using FQ PCR, our aims were to figure out the role of breast milk in transmission of human cytomegalovirus (HCMV) infection, and to compare the FQ PCR and normal PCR assay. Methods: A dual labeled probe hydrolysis FQ PCR method was used to detect HCMV DNA in breast milk of 76 mothers of infants with HCMV infection. We used FQ PCR and normal PCR assays to detect HCMV in urine of 94 subjects. Results: Thirty subjects had posi tive infection of HCMV and 36 negative with both FQ PCR and normal PCR assays. The positive rate of FQ PCR was 60.6%(57/94), and that of normal PCR was 33.0%(31/94). The uniformity rate of both methods was 70.2% (χ 2=25.3>6.6,P<0.01). FQ PCR assay was superior to normal PCR (χ 2=23.3>6.6,P<0.01). With FQ PCR, 57 mothers (72.37%) were HCMV positive in breast milk. Conclusion: Detection of HCMV in breast milk with FQ PCR is sensitive and convenient. Breast milk is important in transmission of HCMV infection.