高压氧对大鼠持续性局灶脑缺血后梗塞灶体积的影响

Effect of Hyperbaric Oxygen on the Infarct Volume After Persistent Focal Cerebral Ischemia in Rats

目的 :探讨高压氧治疗对大鼠局灶性脑缺血的作用及机制。方法 :应用线栓法制作大鼠局灶性脑缺血模型。随机分为假手术组 ,缺血对照组 ,缺血 +高压氧组 ,缺血 +高压空气组。每组各分 5小组 (n =4 ) ,分别于 6,2 4 ,4 8,72h及 7d断头。观察各组大鼠梗塞灶体积的大小 ,并进行组间比较。结果 :高压氧组 7d大鼠梗塞灶体积 (180 .3± 15 .5 )mm3 明显小于缺血对照组 (2 2 1.3± 17.1)mm3 (P <0 .0 1)。而高压空气组与缺血对照组比较 ,差异无显著性。结论

Objective: Our aim was to investigate the effect and mechanism of hyperbaric oxygen (HBO) on brains of rats after persistent focal cerebral ischemia. Methods: Rat models of persistent focal cerebral ischemia were induced by intraluminal filament occlusion to the middle cerebral artery. Rats were randomly divided into sham operation group, ischemic group, ischemia+HBO group, and ischemia+hyperbaric air (HA) group. Each group was further divided into five small groups (n=4), in which rats were killed at 6, 24, 48, 72 hours, and 7 days after ischemia onset and the brain tissues were taken out for histological analysis of infarct volume. Results: Seven days after ischemia onset, there is remarked difference on the infarct volume between the HBO group\[(180.3±15.5)mm 3\] and the control group \[(221.3±17.1) mm 3\] (P<0.01); but no difference was found between the HA group and the control group. Conclusion: Early use of HBO may be beneficial to focal cerebral ischemia.