血管内皮细胞的活化有利于造血干细胞的归巢

Activation of vascular endothelial cells improves the homing of hematopoietic stem cells

目的 :探讨影响造血干细胞归巢的因素 ,为提高造血干细胞移植的效率奠定基础。方法 :传代培养的血管内皮细胞长成单层后 ,用血管内皮细胞生长因子 (VEGF)、粒细胞集落刺激因子 (G -CSF)和脂多糖 (LPS)激活内皮细胞 ,再将经过去除红细胞、粒细胞、单核细胞和T淋巴细胞而富集的脐血造血干细胞与内皮细胞共同培养 ,使之发生粘附。用双抗体夹心ELISA法测定C -Kit+ 的造血干细胞与血管内皮细胞的粘附情况。并比较VCAM - 1单克隆抗体封闭血管内皮细胞对造血干细胞粘附的影响。结果 :静息状态的血管内皮细胞能够与C -Kit+ 的造血干细胞发生粘附 ,但粘附率较低。用VEGF、G -CSF和LPS激活血管内皮细胞后 ,造血干细胞的粘附显著增加。用粘附分子VCAM - 1单克隆抗体预处理激活血管内皮细胞后 ,造血干细胞的粘附明显下降。结论 :激活的血管内皮细胞明显增加对造血干细胞的粘附 ,这种粘附与粘附分子的作用有关。

AIM: To study the effect of endothelial cell activation on the homing of hematopoietic stem cells (HUHSC) during transplantation. METHODS: Human umbilical vein endothelial cells (HUVEC) were cultured to single layer and activated by vascular endothelial growth factor (EVGF),granulocyte colony stimulating factor (G-CSF) and lipopolysaccharide (LPS),respectively. The HUHSC,enriched by eliminating red blood cells,granulocytes,monocytes and lymphocytes from cord blood,were cocultured with activated HUVEC to make adhesion. The adhesive ability of activated HUVEC to C-Kit + HUHSC was assayed by ELISA. Anti-VCAM-1 monoclonal antibody was used to detect the effect of activation on HUVEC and HUHSC interactions. RESULTS: Resting HUVEC had a little adhesive ability to HUHSC. A great enhancement of adhesive ability was showed when HUVEC was activated by VEGF,G-CSF and LPS. In the presence of anti-VCAM-1,the adhesive ability of activated HUVEC was decreased remarkablely. CONCLUSION: HUHSC homing may be related to the activation of endothelial cells and adhesion molecules.