脑热清对内毒素性发热家兔下丘脑、脑脊液cAMP及腹中隔区AVP含量的影响

Effect of Nao-re-qing oral liquid on cAMP content in hypothalamus and CSF, and AVP content in ventral septal area of endotoxininduced febrile rabbits

目的:探讨脑热清(NRQ)口服液的解热机制.方法: 复制家兔内毒素(ET)性发热模型,观察NRQ对家兔体温的影响;用放射免疫法检测下丘脑和脑脊液(CSF)中cAMP及腹中隔区AVP含量的变化.结果: (1)NRQ+ET组的ΔT[(0.82±0.08) ℃]、TRI6(5.73±0.09)、下丘脑cAMP含量[(0.70±0.50)nmol/g]、CSF中cAMP含量[(56.86±1.34)nmol/L]及腹中隔区AVP含量[(11.91±3.47)ng/g], 分别低于ET组的ΔT[(1.80±0.16) ℃]、TRI6(11.31±0.20)、下丘脑cAMP含量[(1.35±0.21)nmol/g]、CSF中cAMP含量[(66.69±1.82)nmol/L]、腹中隔区AVP含量[(30.80±9.59)ng/g],两者相比有显著差异(P<0.01).(2)4组的体温变化分别与下丘脑和CSF中cAMP以及腹中隔区AVP的变化呈正相关(下丘脑:r=0.899,P<0.05; CSF:r=0.991,P<0.01;AVP:r=0.972,P<0.01).结论: NRQ的解热机制可能是通过抑制下丘脑cAMP的生成与释放,同时通过促进腹中隔AVP释放两种途径发挥作用.

AIM: To explore the mechanism of Nao-re-qing oral liquid (NRQ) decreasing endotoxin (ET)-induced fever in rabbits. METHODS: (1) The ET-induced fever model was established in rabbits. Febrile response of rabbits was observed. (2) The arginine vasopressin (AVP) content in the ventral septal area (VSA),and cAMP content in hypothalarmus (HP) and CSF were determined by radioimmunoassay.RESULTS: (1) In ET group,the maximal increment in body temperature (ΔT) [(1.80±0.16) ℃],6 h thermal respone index (TRI_6)(11.31±0.20),the cAMP content in the HP [(1.35±0.21)nmol/g],the cAMP content in CSF [(66.69±1.82) nmol/L] and AVP content in the VSA [(30.80±9.59)ng/g ] were significantly higher than those in NRQ+ET group[ΔT(0.82±0.08) ℃,TRI_6(5.73±0.09),HP: cAMP(0.70±0.50)nmol/g,CSF: cAMP(56.86±1.34),AVP:(11.91±3.47)ng/g]( P <0.01). (2) The AVP content in VSA,and cAMP content in HP and CSF were separately paralleled with the fluctuation of body temperature (AVP: r=0.972,P<0.01 ; HP: cAMP r=0.899,P<0.05 ; CSF: cAMP r=0.991,P<0.01). CONCLUSION: The antipyretic action of NRQ may be due to inhibiting the increase in cAMP in HP and meanwhile promoting the release of AVP in VSA.