稀释液对禽流感HA和HI试验影响的分析

The Effect on the Dilution for HA and HI of Avian Influenza

针对稀释液对禽流感血凝及血凝抑制试验的影响进行了试验研究。结果显示,用pH7.0的PBS缓冲液测定抗原的血凝滴度比常用生理盐水和调pH至7.0的生理盐水高出将近一倍,测定的阳性血清的抗体滴度高出一孔。试验表明用PBS为稀释液能够降低试验成本、提高检出率。该研究为标准化禽流感血凝及血凝抑制试验提供了可靠的实验依据。

The effect on the dilution for HA and HI of Avian influenza was analyzed in this study.The results showed that the titers of HA and HI test with PBS(pH7.0)were as twice high as the samples with solution of0.9%Nacl(pH7.0).It showed that it cost less experimen tal expense and had high er positive rate by PBS.The con vinced experiment data was pro vided to stan dard ize the test of HI and HA of AI in this study.