中国狂犬病疫苗CTN-1-V株糖蛋白基因的克隆及序列分析

Cloning and Sequencing of Glycoprotein Gene of Chinese Rabies Vaccine Strain CTN-1-V

目的 研究CTN-1-V株糖蛋白(GP)基因结构特性。方法 利用RT-PCR反应从感染CTN-1-V病毒的Vero细胞中获得精蛋白全长cDNA片段,并克隆至PCR2.1载体,进行序列测定。结果 CTN-1-V株糖蛋白cDNA序列长度为1 575个核苷酸,编码524个氨基酸。与国外已测定的相应序列进行同源性比较,其核苷酸同源性为80.8％～92.4％,氨基酸同源性为82.9％～93.3％。结论 进一步了解CTN-1-V株的基因结构,为筛选特异性疫苗株提供理论依据。

Objective To study the structure of glycoprotein (GP) gene of Chinese rabies vaccine strain CTN-1-V. Methods Amplify the full-length cDNA fragment of GP from the Vero cells infected with CTN- 1-V strain by RT-PCR and clone into PCR2.1 vector for sequencing. Results The cDNA of GP of CTN-1-V strain consisted of 1 575 nucleic acids encoding 524 amino acids. The homologies of nucleic acid and ami-no acid sequences to those reported abroad were 80.8%-92.4% and 82.9%-93.3% respectively. Conclusion The study provided a theoretical basis for selecting a candidate vaccine strain.