摘要
目的 探讨转化生长因子 β1(transforminggrowthfactor β1,TGF β1)及其信号蛋白Smad2 ,3在诱导大鼠心肌细胞肥大中的作用。方法 培养新生大鼠心肌细胞 ,用不同剂量TGF β1刺激 ,检测 [3 H] Leu掺入量 ,RT PCR检测信号蛋白Smad2 ,3mRNA的表达。结果 不同剂量的TGF β1均能明显增加心肌细胞 [3 H] Leu掺入量 ,TGF β1增加肥大心肌细胞Smad2 ,3mRNA的表达。结论 TGF β1能诱导大鼠心肌细胞肥大 ,信号蛋白Smad2 ,3在其中发挥了重要作用。
Objective To investigate the effects of transforming growth factor*.β 1 and signal protein Smad2,3 on rat cardiocyte hypertrophy. Method Cultured neonatal cardiocytes were incubated with different dose TGF*.β 1 ,the incorporation of [ 3H]*.Leucine were measured to jude the hypertrophy,RT*.PCR was used to determine the expression of Smad2,3mRNA. Results Differtent dose TGF*.β 1 could significantly increase [ 3H]*.Leucine incorporation,and the expression of Smad2,3mRNA was increased as well. Conclusion TGF*.β 1 could induce rat cardiocyte hypertrophy,signal protein Smad2,3 had important role in the progression.
出处
《现代医学》
2003年第5期301-304,共4页
Modern Medical Journal
