摘要
目的:研究大肠埃希菌对氟喹诺酮类药物的耐药机制。方法:对大肠埃希菌标准菌44302和临床收集的3株对氟喹诺酮类药物高度耐药的大肠埃希菌及1株敏感菌,用PCR方法扩增DNA旋转酶gyrA基因的N末端编码区,并进行克隆和序列测定。结果:对氟喹诺酮类药物高度耐药的大肠埃希菌的gyrA基因的序列分析,发现3个导致氨基酸改变的基因点突变:丝氨酸-83→亮氨酸;丙氨酸-84→脯氨酸;天冬氨酸-87→天冬酰氨。结论:对氟喹诺酮类药物高度耐药的大肠埃希菌的DNA旋转酶A亚单位存在着基因点突变。
Objective:To study the mechanism of resistance of Escherichia coli to fluoroquinolones.Methods:Escherichia coli standard strain 44 302,three clinical highly fluoroquinolone resistant isolates and one sensitive isolate were included in the study.The N terminal coding region of gyr A gene was amplified by PCR,then cloned and sequenced.Results:DNA sequence analysis of gyr A gene revealed three mutations that resulted in amino acid changes:Ser 83→Leu,Ala 84→Pro,Asp 87→Asn.Conclusion:Mutations of gyr(ase)A gene subunit existed in fluoroquinolone resistant Escherichia coli.
出处
《浙江大学学报(医学版)》
CAS
CSCD
1999年第1期1-3,共3页
Journal of Zhejiang University(Medical Sciences)