我国汉族正常人群血管紧张素转换酶基因多态性的观察

DNA Polymorphism of Angiotensin Converting Enzyme Gene in Chinese Han Nationality

目的：探讨我国汉族人群血管紧张素转换酶（ＡＣＥ）基因多态性的分布特点。方法：应用聚合酶链反应研究了１５９名我国汉族正常人群ＡＣＥ基因多态性分布情况。ＡＣＥ基因１６内含子内２８７ｂｐ片段的存在或缺失作为多态性的标志。结果：我国汉族正常人ＡＣＥ的ＤＤ、ＩＤ、ＩＩ基因型频率分别为０．１５７、０．５３５和０．３０８；与国外其他种族人群比较，我国汉族正常人ＤＤ基因型频率显著低于日本人、白种人和黑种人（Ｐ均＜０．０５）。结论：本研究提示，我国汉族正常人ＡＣＥ基因Ｉ／Ｄ多态性分布不同于国外其他种族，我国汉族人ＤＤ基因型频率低于日本人、白种人和黑种人。

Objective:To investigate the distribution of angiotensin converting enzyme(ACE) gene polymorphism in Chinese Han nationality.Methods:ACE gene polymorphism of 159 unrelated healthy individuals was studied by means of polymerase chain reaction(PCR).The presence or absence of a 287 bp fragment in intron 16 of the gene serves as a marker for this polymorphism.Results:All subjects were genotyped for ACE polymorphism;the frequencies of the DD,ID and II genotypes were 0.157,0.535 and 0.308 respectively.In the Han nationality,the frequency of the DD ACE genotype was lower than that in European,North American,or Japanese subjects( P <0.05).Conclusion::These results suggest that there are significant differences in the distribution of ACE gene between the Han nationality and other ethnic population.