摘要
目的：探讨分离脐血造血干细胞的最佳方法。方法：采用Ｆｉｃｏｌ分层法、３％明胶自然沉降法及６％羟乙基淀粉自然沉降法。结果：３％明胶自然沉降法所获有核细胞（ＮＣ）密度高达９４．６０（±２３．８０）×１０５／ｍｌ，ＣＦＵＧＭ数为６４．３０（±１２．１８）／１０５ＮＣ，ＣＤ３４＋细胞数为１．２１（±０．３７）×１０５／ｍｌ，均较其它两种方法为高（Ｐ＜０．０５）。结论：３％明胶自然沉降法是一种较理想的分离脐血造血干细胞的方法。
Objective: To find out an optimal method of separating
hematopoietic stem cells (HSC) from umbilical cord blood. Methods: Density gradient
centrifugation(FicollHypaque),3% gelatin natural sedimentation and 6% hydroxyethyl starch
natural sedimentation were compared. Results: The number of nucleate cells,CFUGM and
CD34+ cells were 94.60(23.80)105/ml,64.30(12.18)/105 NC and 1.21(0.37)105/ml respectively by
3% gelatin natural sedimentation.These results were better than that from the other two methods
(P<0.05). Conclusion: Three per cent gelatin natural sedimentation is an optimal umethod for
separating HSC from umbilical cord blood.
出处
《浙江大学学报（医学版）》
CAS
CSCD
1999年第3期114-116,共3页
Journal of Zhejiang University(Medical Sciences)
关键词
脐血
造血干细胞
细胞分离
CD34
肝素
Umbilical cord blood
CD34
Hematopoietic
cell
Cell separation
