药物对牛晶体上皮细胞生长的抑制作用

Diclofenac Sodium and Cyclosporin A Inhibit Epithelial Cell Proliferation of Bovine Lens in Culture

目的：研究双氯灭痛钠及环孢霉素Ａ在体外对牛晶体上皮细胞（ＢＬＥＣ）增殖的影响。方法：牛晶体上皮细胞原代培养。用第四代晶体上皮细胞种入２４ 孔板，分别加入不同浓度的双氯灭痛钠及环孢霉素Ａ，２４ ｈ 及７２ ｈ 后用Ｇｉｅｍ ｓａ 法检测细胞增殖抑制率。结果：双氯灭痛钠（≥７５ μｇ／ｍ ｌ）及环孢霉素Ａ（≥２５ μｇ／ｍ ｌ）在体外有抑制晶体上皮细胞增殖的作用。

Objective:To investigate the effect of diclofenac sodium and cyclosporin A on bovine lens epithelial cell proliferation in cultrue.Methods:Bovine lens epithelial cells (BLEC) were cultured in vitro.The fourth passenge cells were seeded on to 24 wells tissue culture plates.After 24 h and 72 h exposure to different concentrations of diclofenac sodium and cyclosporin A,proliferation inhibit rates were estimated by Giemsa method.Results:Diclofenac sodium and cyclosporin A (at concentration≥75 μg /ml and ≥25 μg/ml respectively) inhibited the proliferation of bovine lens epithelial cell in culture in a dose dependent manner.Conclusion:Diclofenac sodium and cyclosporin A at appropriate dose are effective in inhibition cultured bovine lens epithelial cell proliferation which may contribute to preventing the posterior capsule opacification.