蜂毒素的抗肿瘤生物活性及其稳定性研究

Study on the Antitumor Activity of Melittin and its Stability

目的:通过中压液相色谱提取、纯化蜂毒素,研究其抗肿瘤生物学活性,及其稳定性。方法:通过中压液相色谱提取蜂毒素,经HPLC检测纯度,采用MTT法,体外观察蜂毒素对4种肝癌细胞系增殖的抑制作用,同时与蜂毒素标准品进行抗肿瘤生物活性的比较,并比较不同批次提纯蜂毒素的抗肿瘤作用。结果:蜂毒素在16-128μg/ml对肝癌细胞增殖有抑制作用,并呈剂量依赖性,对人正常肝脏来源细胞株L-02细胞抑制作用不明显。在60μg/ml浓度,各批次提取的蜂毒素与蜂毒素标准品间抗肿瘤活性无显著性差异。结论:采用中压液相色谱提取、纯化的蜂毒素具有抗肿瘤生物活性,并且稳定性良好。

Objective :To study the effect of melittin on antitumor activity and its stabiltity in vitro. Methods: Human hepatoma-de-rived cell lines and human hepatocyte-derived cell lines were treated with melittin.The proliferation inhibition was observed by MTT assay, and its stability on extraction and purification from different time was estimated. Results: The data showed that melittin could inhibit the proliferation of human hepatoam-derived cell lines dose-dependently at 16 and 128μg/ml.The antitumor activity had no significant difference between the extraction and purification melittin from different time and standard melittin at 64μg/ml concentration of melittin for treated cells respectively. Conclusion: Melittin inhibited the proliferation of hepatoma cell lines with good stability.