一氧化氮在神经根性疼痛中的作用

Effects of Nitric Oxide on Radiculopathy due to Herniated Nucleus Pulposus of the Lumbar Spine

目的 :探索一氧化氮 (NO)在髓核突出所致的神经根性疼痛中的作用。方法 :取大鼠自体尾椎髓核无压迫下放置在L4 和L5神经根表面 ,分别在术后 3d及 1、 2、 3、 4周时观察大鼠后足机械刺激和热刺激敏感性的变化 ,并用免疫组化方法对移植髓核中的一氧化氮合酶 (NOS)进行检测 ,探索NO在疼痛中的作用。结果 :在无明显机械压迫情况下 ,大鼠腰神经根上放置自体髓核可产生痛觉过敏 ,移植髓核组织中NOS染色阳性。结论 :髓核自身是引起腰腿痛的重要原因 ,NO可能参与疼痛的产生。

Objective:To determine whether or not nitric oxide(NO) is involved in the pathophysiological mechanism of radiculopathy caused by herniated nucleus pulposus in lumbar spine.Methods:The nucleus pulposus from coccygeal vertebra was applied to the L 4 and L 5 nerve roots thout compression and mechanical threshold for foot withdrawal and thermal foot withdrawal latency were observed in 3 days, 1 week, 2 weeks, 3 weeks and 4 weeks after surgery respectively and immunohistochemical method was introduced to detect nitric oxide synthase(NOS) in the applied tissue. Results:The rats showed obvious hyperalgesia after application of autologous nucleus pulposus on the nerve root without compression in the operated limb and immunohistochemical staining showed that NOS were positive in the applied tissues. Conclusions:The biologic effect of nucleus pulposus is an important factor in the pathogenesis of low lumbar and leg pain and NO may play some role in the pathogenesis of pain.