化学发光法检测肾移植患者吞噬细胞的吞噬功能

Estimation in Phagocytic Function in Phagocytes in Renal Transplanted Patients by Chemiluminescence(CL)

用化学发光法(CL)检测了4组患者和正常对照组吞噬细胞的吞噬功能;进行了吞噬细胞吞噬功能CL峰值/10~3白细胞,体内吞噬细胞预激活状态(本底),血浆调理活性的比较。结果肾移植术后肾功能良好的受者CL峰值/10~3白细胞与正常组比较无显著差异(P>0.05)。术后肾功能较差的受者、未移植透析的患者和术后因排异已切除移植肾的患者CL峰值/10~3白细胞与正常组有显著差异(P<0.05),本底水平比正常对照组显著升高,峰时与正常组无差异。结果表明,肾移植术后吞噬功能的增强,体内预激活状态增高,预示着肾功能减退、排异出现。这一结果对诊断肾移植术后排异具有一定的参考价值。

The changes of phagocytic function in phagocytes were estimated by chemiluminescence (CL). in renal transplanted patients prior and after their surgery. Forty-seven patients re- ceived renal transplantation for 1 to 8 years, (thirty-seven with normal renal function and 10 with abnormal renal function), were randomly chosen to be the subjects for estimating the peak CL/10~3 leucocytes, the state of pre-activated phagocytes in vivo(basal) and the opsonic activity of plasma, comparing with 15 hemodialysis patients who had not received transplan- tation, 13 renal transplanted patients whose transplanted kidney had been removed due to se- vere rejection, and 38 normal controls. The peak CL/10~3 leucocytes of receptors with good renal function had no apparent difference compared with that of normal control, while The peak CL/10~3 leucocytes of the receptors with abnormal renal function significantby differed from the value of control, dialysis patients without transplantation and patients whose trans- planted kidney removed (P<0. 05). In comparing with the controls, the backgrand level in patients obviously increased while no difference was shown in the peak time. These results show that after renal transplantation the increasments of phagocytic function and the pre-ac- tivated state in vivo predict the occurence of renal function failure and rejrction. This study suggest that is useful in diagnosing the rejection of transplant in clincal examination.