摘要
用蔗糖密度梯度离心法提纯鸡病毒性关节炎病毒(AVAV)作包被抗原,建立了间接ELISA的最佳工作程序,其中抗原包被浓度为0.61μg/孔,被检血清为1:50.酶结合物1:1600;其灵敏度为琼脂凝胶沉淀试验(AGP)的238.5倍.在受检的529份鸡血清中,该法检出率(85.1%)显著高于AGP(46.0%).间接ELISA对S1133疫苗及AVAV—J株接种鸡抗体消长情况的观察结果表明,S1133疫苗常量接种后所产生的免疫力足以抵抗强毒株AVAV—J的攻击,AVAV—J毒株的良好免疫原性.使其有可能成为研制国产疫苗的候选毒株.
The avian viral arthritis virus (AVAV) purified by sucrose density gradient cen-trifugation was used as coated antigen. An optimum procedure of indirect enzyme-linked im-munosorbent assay (ELISA) was developed to detect the antibody against AVAV. The concentration of coated antigen is 0. 6 μg per well, dilution of sera detected 1 : 50 and that of the enzyme conjugate 1 : 1600. The results showed that the sensitivity of the ELISA is 238. 5 times as high as the agar gel precipitation (AGP) test, and its detectable rate (85. 1 % ) is much higher than AGP (46.0%) .
