兰州地区无偿献血人群血清TTV-DNA的检测

Detection of transfusion transmitted virus in serum samples of the blood donors in Lanzhou region

为了解兰州地区人群TTV的感染状况 ,作者采用套式PCR法 ,以TTV DNA的ORF1中的一段 30 9nt的序列为扩增序列 ,对 36份兰州地区无偿献血人群血清DNA进行扩增 ,将扩增阳性片段测序并进行序列同源性比较 ,结果发现兰州地区无偿献血人群TTV感染率为 5 2 .7%。 10份兰州序列之间的同源性为 88.6 %～ 95 .4 % ,兰州序列与日本TA2 87株的同源性为 88.1%～ 93.3% ,表明兰州地区无偿献血人群TTV感染非常普遍 ,兰州地区TTV流行株存在变异 ,至少有基因亚型存在。

In order to detect the transfusion transmitted virus (TTV) DNA in serum samples of the voluntary blood donor in lanzhou region , we amplified a 309nt DNA of TTV ORF1 by nest PCR.The result showed that the TTV-DNA was detected in 19 of 36 donors with 52.7% positive rate. The nucleotide sequence homology of lanzhou TTV isolates were ranged from 88.6%～95.4%,and the nucleotide sequences of lanzhou TTV isolates were 88.1%～93.3% analogous to the Japanese isolated TA287. We draw a conclusion that TTV infection is common in voluntary blood donors of lanzhou region,and at least there exist subgenotype of TTV in lanzhou region.