白色力克斯兔、哈尔滨白兔及杂交后代血液蛋白多态性研究——Ⅰ.电泳图谱及多态性蛋白的基因分布

Studies on the Blood Protein Polymorphism in White Rex Rabbit, Harbin White Rabbit and Their Filial-Generations Ⅰ. Electrophoretic Patterns and Gene Distribution of Polymor- phic Protein

利用垂直板聚丙烯酰胺凝胶电泳法对白色力克斯兔(WR)、哈尔滨白兔(HW)及其杂种一代(F_1,♂WR×♀HW)和回交一代[B_1,♂WR×♀F_1包括分离出的力克斯毛型(B_1R)和正常毛型(B_1N)]血液中的红细胞酯酶(Es—1、Es—2和Es—3)、血清转铁蛋白(Tf)、前转铁蛋白(Prt)、后白蛋白(Po)、血红蛋白(Hb)、血清过氧化氢酶(Cat)、血清过氧化物酶(Pod)和红细胞乳酸脱氢酶(LDH)等10个蛋白位点进行了测定.结果表明,Hb、Cat、Tf和LDH呈单态,其他位点呈多态.在多态位点中,Es—1、Es—2、Es—3、Po和Prt分别出现3,3,3,3和6种表型,受2,2,2,2和3个共显性等位基因控制.Pod共有5种表型,在WR群体中只有表型Ⅰ呈单态.x^2检验表明,哈尔滨白兔在Es—1、Es—2、Es—3、Po和Prt5个位点上均处于平衡状态,而其他群体则不同程度出现不平衡状态.

Ten protein loci, including erythrocyte esterase (Es-1, Es-2 and Es-3) , transfer-rin (Tf), pretransferrin (Prt), postalbumin (Po), haemoglobin (Hb), peroxidase (Pod), cata-lase (Cat) and lactate dehydrogenase (LDH) were detected by vertical plate polyacrylamide gel electrophoresis in blood samples from white Rex rabbit (WR), Harbin white rabbit (HW) and their first filial generation (F1 WR × HW) , first backcross generation [B1 WR × F1 , including segregated Rex coat rabbit (B1R) and normal coat rabbit (B1N)] . The results showed that 4 protein loci (Hb, Cat, Tf and LDH) are monomorphic, but the others (Es-1, Es-2, Es-3, Po, Prt and Pod) are polymorphic. The polymorphisms of Es-1, Es-2, Es-3, Po and Prt are controlled by 2, 2, 2, 2, and 3 codominant alleles and exhibit 3, 3, 3, 3 and 6 phenotypes respectively. 5 phenotypes of Pod can be expressed, but only phenotype I (monomorphism) in WR. All gene distribution of Es-1, Es-2, Es-3, Po and Prt lay in Hardy - Weinberg equilibrium in HW, whereas the loci of F1 , B1R and B1N (X2 test) in WR are in disequilibrium.