实验性鸡病毒性关节炎病理学研究

Pathological Studies on Experimental Viral Arthritis in Broilers

应用国内分离的鸡病毒性关节炎病毒“吉毒株”(VAV—J)成功地复制了鸡病毒性关节炎(VA).详细观察了实验性鸡病毒性关节炎的临床症状,并运用肉眼、光镜、电镜及免疫琼脂扩散等技术对该病进行了系统的病理学研究.结果表明,实验性鸡病毒性关节炎腱断裂病变少见;急性期,关节与腱鞘滑膜下网状细胞增殖,大量异染性细胞、淋巴细胞和巨噬细胞浸润,有的严重出血;慢性期,滑膜形成绒毛样突起,并有淋巴细胞性结节形成,并见籽骨骨化障碍;未发现以异染性细胞浸润为主的心肌炎;免疫器官具有明显病变.关节液中病毒粒子的检出率为100%,可用于本病的诊断.

Avian viral arthritis was produced experimentally in broilers by inoculating viral arthritis virus Jilin strain (VAV -J). The clinical manifestations of experimental cases were observed and the systemic pathology was studied by naked eyes, light - microscope, electron -microscope , etc. The results showed that rupture of tendons seldom occurred. In the acute phase, there was multiplication of reticular cells in synovial membranes of tendon sheathes and joints, and accompanied by infiltration of a lot heterophils, lymphocytes and macrophages. In'the chronic phase, the synovial membranes formed villous projections and lymphoid nodes. The ossification of sesamoid bones was hindered. The lesion of myocarditis characterized by infiltration of heterophils had not been observed. The pronounced lesions were seen in the immunological organs. By electron - microscopic examination of nagative-stained preparations of synovial fluids, virions were found in all cases.