摘要
AIM: To investigate the expression of c-myc target from laryngeal cancer cells (MTLC) gene in gastric carcinoma (GC)tissues and the effect of MTLC over-expression on gastric carcinoma cell line BGC823.METHODS: RT-PCR was performed to determine the expression of MTLC mRNA in GC and matched control tissues.BGC823 cells were transfected with an expression vector pcDNA3.1-MTLC by liposome and screened by G418. Growth of cells expressing MTLC was observed daily by manual counting. Apoptotic cells were determined by TdT-mediated dUTP nick-end labeling (TUNEL) assay.RESULTS: The expression of MTLC mRNAs was downregulated in 9(60%) of 15 cases of GC tissues. The growth rates of the BGC823 cells expressing MTLC were indistinguishable from that of control cells. A marked acceleration of apoptosis was observed in MTLC-expressing cells.CONCLUSION: MTLC was down-regulated in the majority of GC tissues and could promote apoptosis of GC cell lines,which suggests that MTLC may play an important role in the carcinogenesis of gastric carcinoma.
AIM:To investigate the expression of c-myc target from laryngeal cancer cells(MTLC)gene in gastric carcinoma(GC) tissues and the effect of MTLC over-expression on gastric carcinoma cell line BGC823. METHODS:RT-PCR was performed to determine the expression of MTLC mRNA in GC and matched control tissues, BGC823 cells were transfected with an expression vector pcDNA3.1-MTLC by liposome and screened by G418.Growth of cells expressing MTLC was observed daily by manual counting,Apoptotic cells were determined by TdT-mediated dUTP nick-end labeling(TUNEL)assay. RESULTS:The expression of MTLC mRNAs was down- regulated in 9(60%)of 15 cases of GC tissues.The growth rates of the BGC823 cells expressing MTLC were indistinguishable from that of control cells.A marked acceleration of apoptosis was observed in MTLC-expressing cells. CONCLUSION:MTLC was down-regulated in the majority of GC tissues and could promote apoptosis of GC cell lines, which suggests that MTLC may play an important role in the carcinogenesis of gastric carcinoma.
基金
the National Natural Science Foundation of China,No.30171008
Funds of Educational Department,Liaoning Province,No.20121034
