香蕉冠腐病病原菌的分离和鉴定

Isolation and Identification of Banana Crown Rot

对海南省东方、乐东、三亚、琼海、儋州和澄迈六市县的香蕉进行抽样调查,对发生典型冠腐病症状的香蕉进行组织分离,培养,统计带菌率和带菌种类,结果显示六个市县香蕉的带菌率为13.3%～87.2%,其中以三亚的最高。在分离到的9个菌株中,镰刀菌6种,经过致病力测定和生物学鉴定,确定引起省内香蕉冠腐病的主要病原菌为亚粘团串珠镰孢(Fusariummoniliformevar.subglutinans)、半裸镰孢霉(F.semitectum)、导管镰孢霉(F.aquaeductuum)和弯喙镰孢霉(F.camptoceras)。

Banana samples from Dongfeng,Ledong,Sanya,Qionghai and Chengmai were col-lected in order to isolate and culture those which showed symptoms of crown rot and to measure the rate and varieties of fungi-carrying bananas.The results showed that13.3%～87.2%carried fungi,and fungi-carrying rate was highest in Sanya;there were6species of Fusarium spp.in isolated nine strains.Based on a pathogenicity and biological identification,a conclusion was drawn that F.moniliforme var.subglutinans,F.semitectum,F.aquaeductuum and F.campto ceras caused the banana crown rot in Hainan province.