巴曲酶对脑缺血再灌注大鼠海马CA1区的影响

Experimental study on the therapeutic effectiveness of batroxobin in hippocampal CA1 region in rat focal cerebral ischemia reperfusion model

目的 通过观察大鼠局灶性脑缺血再灌注不同时段 ,巴曲酶对海马CA1神经元及星形胶质细胞数目、形态等方面的影响 ,从而探讨巴曲酶对局灶性脑缺血再灌注损伤的保护作用。方法 采用改良的线栓法制备大脑中动脉阻塞 (MACO) 2h、不同再灌注时间段 (3h、6h、12h、2 4h、48h、72h、7d)的大鼠短暂局灶性脑缺血 (transientfocalcerebralischemia)模型 ,随机设立巴曲酶组 (Bat)、生理盐水对照组 (N .S)、假手术组 (sham operated) ,通过HE染色及胶质原纤维酸性蛋白 (GFAP)和神经元特异核抗原 (NeuN )的免疫组化染色 ,观测CA1区神经元和星形胶质细胞的形态、数目的动态变化。结果 巴曲酶能显著提高再灌注早期 (6～ 2 4h)CA1区GFAP阳性细胞的数目 ,再灌注 7d组存活锥体细胞的数量较盐水对照组有明显提高 ,提示局灶性脑缺血后早期反应性星形胶质细胞的增多对维持神经元的存活有积极意义 ,巴曲酶对短暂局灶性脑缺血再灌注引起的海马CA1区延迟性神经元坏死 (DND)

Objective The present study was conducted to evaluate the effects of batroxobin on morphol ogical features and on the amount of GFAP positive cells and survival pyramidal neurons in hippocampal CA1 region after transient focal cerebral ischemia. Methods Focal cerebral ischemia was induced in male Sprague Dawley rats (weight 250 30 0 g) by transient occlusion of the right middle cerebral artery (MCAO) using an intraluminal thread model. All animals were randomly devided into batroxobin tre ated group (Bat),normal saline control group (N.S) and sham operated group (Sha m)and subjected toischemia for 2 h, followed by 3h,6h,12h,48h,72 h,7d of reperfusion.The slices were stained with H E and with NeuN&GFAP immunoh istological double staining. Consults Batroxobin increased significa ntly the numbers of GFAP positive cells in CA1 region in earlier periods of repe rfusion (3 24h).The number of survival pyramidal neurons wasmuch more t han that in N.S group.ConclusionThe increase of reactive a strocytes in earlier periodsimplies that astrocytes positively respond t o the neuronal injuries, which might play a role in promoting neuronal survival. This study indicates that batroxobin provide neuroprotection by preventing delay ed neuron death (DND)after transient focal cerebral ischemia