不同染色方法对牛卵泡AP和G-6-P酶染色效果的影响

The Effects of Different Staining Method on AP and G-6-P Staining Results in the Cattle Ovary Follicles

应用组织化学手段对牛卵巢卵泡中碱性磷酸酶(AP)和葡萄糖-6-磷酸酶(G-6-P酶)进行酶活性反应显示和定位。旨在探讨不同染色方法对酶活性反应显示的影响,以便更好的观察不同发育时期卵泡内酶的定位和酶活性反应的强弱。结果表明:AP检测效果实验Ⅰ组好于实验Ⅱ组和对照组,其卵巢组织结构及卵泡形态非常清晰,切片背景为兰色,酶活性处呈现黑色沉淀,各期卵泡酶的反应显示较好。G-6-P酶检测效果实验Ⅱ组好于实验Ⅰ组和对照组,其卵巢组织结构及卵泡形态比较清晰,切片背景为浅粉色,酶活性反应处呈现棕色沉淀,酶活性反应显示较好。说明不同染色方法对酶活性反应观察效果不同。牛卵巢卵泡不同发育时期、不同的结构部位,酶的活性具有强弱不同的反应性。

Enzyme activity reaction and location of alkaline phosphatase(AP)and glucose-6-phosphatase(G-6-P)were observed by histochemical method in cattle ovarian follicles.The results showed that expⅠgroup was better than ExpⅡgroup and control group in the staining of AP,however,expⅡgroup was better than expⅠgroup and control group in the staining of G-6-P.The results of enzyme activity reaction differed in different staining methods.The enzyme activity had different strong and weak reaction in the different structural position of follicles and different developmental stage follicles from cattle ovary.