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噬菌体抗体库技术及其研究策略 被引量：1

Phage Antibody Library Technology and Its Research Strategies
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摘要 噬菌体抗体库技术是近年发展起来的将抗体基因与噬菌体外壳蛋白基因在其表面进行融合表达的技术。可用来构建噬菌体抗体库 ,并可从中制备各种基因工程抗体 ,为抗体的进一步开发应用提供了具有良好前景的新方法。 Phage antibody library is a new technology through which antibody genes were linked with phage coat protein genes, and the fused genes were cloned and expressed on the surface of phage. Phage antibody library can be constructed and various kinds of genetically engineered antibodies can be produced. It provides a new means for antibody development and application.
作者 苗向阳
机构地区 中国农业科学院畜牧研究所
出处 《中国畜牧杂志》 CAS 北大核心 2003年第6期44-45,61,共3页 Chinese Journal of Animal Science
基金 国家自然科学基金资助项目 (No.30 0 70 543) 北京市自然科学基金资助项目 (No .50 32 0 1 2 )
关键词 噬菌体 抗体库技术 兽医科学 基础学科 基因工程 Basal disciplines of animal and veterinary science Phage antibody library technology A review Genetic engineering Antibodies
分类号 S852.43 [农业科学—基础兽医学]
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参考文献11

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同被引文献24

  • 1苗向阳,丁淑燕,朱玉良,郝慧芳,邵建军.噬菌体抗体库的构建及抗猪脂肪细胞膜单抗的筛选[J].中国农业科学,2005,38(6):1260-1263. 被引量：5
  • 2Hoogenboom, H R. Overview of antibody phage-display technology and its applications. Methods in Molecular Biology, 2002, 178: 1-37.
  • 3Honon R M, Cai Z, Ho S N, Pease L R. Gene splicing by overlap extension: tailor-made gene using the polymerase chain reaction. Biotechniques, 1990, 8(5): 528-535.
  • 4Hoogenboom H R, Bruine A P, Hufton S E, Hoet R M, Arends J W, Roovers R C. Antibody phage display technology and its applications. Immunotechnology, 1998, 4(1): 1-20.
  • 5Vaughan T J, Osbourn J K, Tempest P R, Human antibodies by design. Nature Biotechnology, 1998, 16: 535-539.
  • 6Benhar I. Design of synthetic antibody libraries. Expert Opinion on Biological Therapy, 2007, 7(5): 763-779.
  • 7Presta L G. Selection, design, and engineering of therapeutic antibodies. Journal of Allergy and Clinical Immunology, 2005, 116(4): 731-736.
  • 8Griffiths A D, Williams S C, Hartley O H, Tomlinson I M, Waterhouse P, Crosby W L, Kontermann R E, Jones P T, Low N M, Allison T J. Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO Journal, 1994, 13(14): 3245-3260.
  • 9Sheets M D, Amersdorfer P, Finnern R, Sargent P, Lindquist E, Schier R, Hemingsen G, Wong C, Gerhart J C, Marks J D. Efficient construction of a large nonimmune phage antibody library: The production of high-affinity human single-chain antibodies to protein antigens. Proceedings of the National Academy of Sc&nces of the United States of America, 1998, 95(11): 6157-6162.
  • 10Vaughan T J, Williams A J, Pritchard K, Osbourn J K, Pope A R, Earnshaw J C, McCafferty J, Hodits R A, Wilton J, Johnson K S. Human antibodies with sub-nanomolar affinities isolated from a large no-immunized phage display library. Nature Biotechnology, 1996, 14(3): 309-314.

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中国畜牧杂志
2003年 第6期

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