癌症患者淋巴细胞及亚群改变和癌细胞CD_(44)含量与肿瘤形成及转移的关系

Relationship between the changes of lymphocyte subsets anti CD_(44) content in cancer cells and carcinogenesis and metastasis

目的探讨癌症患者细胞免疫状况、NK细胞及癌细胞CD44含量与肿瘤形成和转移的关系 ,评价细胞免疫、NK细胞和癌细胞CD44含量检测在恶性肿瘤诊断、浸润转移潜能及预后判断中的作用。方法采用流式细胞仪检测癌症患者细胞免疫功能及手术切除的癌组织及癌旁正常组织细胞CD44定量检测。结果癌症患者全组CD3 、CD4、CD4/CD8、CD16+ 56都显著低于正常对照组 ,有淋巴结转移组又明显低于无淋巴结转移组。CD8有淋巴结转移组明显高于无淋巴转移组 ,CD19细胞在各组间无显著性差异。CD44在癌组织组明显高于癌旁周围正常组织组 ,CD44在有淋巴结转移组和无淋巴结转移组中有极显著差异。结论癌症患者细胞免疫功能、NK细胞数及癌细胞CD44含量与肿瘤形成、转移有关 ,检测癌症患者细胞免疫、NK细胞数及癌细胞CD44含量对临床诊断、预测肿瘤转移和判断患者预后起重要作用。

Objective To explore the relationship between lymphocyte immune function and CD 44 content in cancer cells and carcinogensis and metastasis.Methods Lymphocyte immune function and CD 44 content of tumor cells were tested by flow cytometry.Results The levels of CD 3、CD 4、 CD 4/CD 8 and CD 16+56 were lower in cancel patients than normal and colorectal cancer patients with lymphnodes metastasis was much lower than that of that of patients without metastasis. The levels of CD 8 of patients with lymphnodes metastasis was higher than that patients without metastasis. CD 44 content in cancers was much higher, than that normal tissue.CD 44 content of patients with lymphnodes metastasis was higher than that patients without metastasis. Conclusion Lymphocyte immune function and CD 44 expression is relate to carcinogenesis and metastasis of the cancer.