大鼠不同方式运动后骨骼肌损伤与血浆CK及其同工酶变化的关系

Relationship between Muscle Damage and Changes of Plasma CK and its Isoenzymes of Rats after Different Modes of Exercise

为了探索大鼠完成不同方式的运动后骨骼肌损伤与血浆CK及其同工酶变化的关系 ,本研究采用了两种动物模型。动物模型一 :雄性SD非训练大鼠分别进行 2 5分钟、50分钟和力竭三个负荷的游泳运动 ;动物模型二 :雄性Wistar大鼠进行为期两周的下坡跑训练 ,最终进行一次 3级递增负荷下坡跑运动。大鼠运动后检测血浆CK及其同工酶活性变化 ,同时取比目鱼肌观察骨骼肌Z线异常变化。结果发现 ,大鼠游泳运动后即刻血浆CK及其同工酶CK -MM %水平与比目鱼肌Z线异常率之间的线性相关系数分别是 0 875(P <0 .0 1 )和 0 47(P <0 0 1 ) ;大鼠下坡跑运动后即刻血浆CK、CK -MM %水平与比目鱼肌Z线异常率的线性相关系数分别是 0 .368(P <0 0 5)和0 379(P <0 .0 5)。结果表明大鼠进行不同方式的运动 (向心与离心收缩 )后即刻骨骼肌的损伤与血浆CK及其同工酶CK -MM

Two animal models were established in this experiment to investigate the relationships between muscle damage and plasma activity of CK and its isoenzymes of rats after different modes of exercise.Model 1: untrained male Sprague-Dawley rats undertook swimming exercise of three different durations:25 minutes, 50 minutes and exhaustion. Model 2:after 2-week′s downhill running, male Wister rats undertook a 3-graded downhill running finally. Plasma and soleus muscle samples were taken to observe the changes of CK, CK isoenzymes and muscle damage. The linear correlation coefficients between plasma CK activity, CK-MM% and the Z-band abnormity rate of soleus muscle immediately after swimming in model 1 were 0.875 ( P <0.01) and 0.47( P <0.01) respectively, and 0.368 ( P <0.05) and 0.379( P <0.05) in model 2 respectively. Results suggested that there was a trend of linear correlationship between plasma CK, CK-MM% and the muscle damage of rats after different mode of exercise (concentric and eccentric contraction).