葡萄糖氧化酶活力的微波技术检测研究

Detection of Glucose Oxidase Activity with Microwave Technology

微波检测技术具有非接触、无损伤、无污染和速度快等特点。葡萄糖氧化酶的生产和使用时必须要考虑到其活力,目前的检测方法或多或少都存在一些不足。微波检测技术可以简化操作和仪器设备、实现无损、连续、智能化检测。利用微带线型微波器件终态检测葡萄糖氧化酶催化体系的反射系数S11。结果表明,反应前后反应体系的反射系数变化值ΔS11在p H4.5到p H6.5范围内先增大后减小,且在p H5.5处具有最大值,这与分光光度法检测结果相同,证明了微带线器件用于检测葡萄糖氧化酶的可行性。随后利用微带线实时监测葡萄糖氧化酶10 min内的酶促反应,发现在54005 440 MHz微波频段,反应体系的S11随着时间的延长而逐渐增大,表明微带线器件能够用于葡萄糖氧化酶酶促反应的实时监测。

Microwave detecting technology has many characteristics such as non-contact,non-intrusion,non-pollution and rapid measurement. It is necessary to detect the activity of glucose oxidase in its production and application. There are a variety of deteced methods at present. However,each of them has more or less advantages and disadvantages. The application of microwave detecting technology in enzyme activity testing can simplify the experimental operation,thus realize non-intrusive,continuous and intelligent detecttion. In this article,a microstrip line device was used as biosensor to detect the reflection coefficient of the reaction solution before and after the enzymatic reaction. The reflection coefficient detected by a microstrip-line device changes with a same trend as the enzyme activity detected by the spectrophotometric method,that is,ΔS11 increases in the range of p H 4.5 to p H5.5,but decreases in the range of p H 5.5 to p H 6.5,which confirms that the microstrip line can be used to detect the activity of glucose oxidase. Then the microstrip line device was used for real-time monitoring of glucose oxidase-catalyzed reaction. The experimetal results show that the reflection coefficient increases with the increase of reaction time in the frequency range from 5400 MHz to 5440 MHz,which once again confirms that a microstrip line device can be used in real-time monitoring of the glucose oxidase reaction.